measurement of cell bound of 1 n phenyl naphthylamine

  • Effect of EDTA on Salmonella enterica serovar Typhimurium

    exponential phase, as indied by uptake of 1-N-phenylnaphthylamine (a nonpolar release (determined by measuring LPS-specific fatty acids or 14C- labelled LPS in cell-free EDTA-released LPS vs cell-bound LPS was identical. It can,.

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  • Surface changes and polymyxin interactions with a - CiteSeerX

    with N-phenyl-1- naphthylamine (NPN) uptake, fluorometric binding and thermal shift assays, lysozyme and deoxycholate sensitivity assays, . polymyxin binding target in the outer membrane of Materials. Polymyxin B (sulfate), colistin (sulfate), N-phenyl-1- . of the cell suspensions were measured, cells were again.

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  • N-phenyl-1 -naphthylamine as a Fluorescent Probe for Early Event

    8 Jun 1982 N-phenyl-1-naphthylamine — Intensity of fluorescence. Introduction The process of killer toxin action involves binding to a cell wall receptor. (Al-Aidroos and Preparation of cell suspension for fluorescence measurements.

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  • 17-AAG - Cancer Research

    1 Nov 2005 of human breast cancer cell lines differing only in NQO1 levels. MDA468 cells Raf-1 degradation were measured by immunoblot analysis. Marked none and the Hsp90 protein, 17-AAGH2 was bound more tightly to the . acetonitrile containing 1 Ag/mL N-phenyl-1-naphthylamine (internal standard).

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  • Comparing bacterial membrane interactions and antimicrobial

    cell-selective antimicrobial peptides with improved an- timicrobial LFP-20 in displacing lipopolysaccharide-bound dansyl- ricidal mechanism by measuring depolarization of the β-d-galactoside (ONPG), N-phenyl-1-naphthylamine.

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  • Calcium-Dependent Protein Phosphorylation May Mediate the

    increase in cytoplasmic [Ca2], and the cell death normally induced by GA, and . et al., 1996) and N-phenyl-1-naphthylamine staining. (Saunders and Hepler 

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  • The interaction of detergents with phospholipid vesicles - FEBS Press

    cell membranes with the so-called fusogenic lipids. [4] showed and N-phenyl- 1 -naphthylamine (NPN) (Aldrich) were used without 1 h at 25°C before carrying out the measurements. . is bound to the bilayer, the liposome concentration.

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  • Interaction of the Fluorescent Dye 1-N-Phenylnaphthylamine with

    Membranes are among the critical targets of heat in bacterial cells (Beuchat, 1- N-Phenylnaphthylamine (NPN), being an uncharged lipophilic dye, . at 5 p~ to the heated cell suspension, the fluorescence was measured as shown in Fig. 1. . molecules continue to enter the cell membranes until the binding sites in them.

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  • The photoirradiation induced p–n junction in naphthylamine-based

    The bulk heterojunction (BHJ) plays an indispensable role in organic such as poly(3-hexylthiophene-2,5-diyl) (P3HT) and phenyl-C61-butyr. p–n heterojunction in naphthylamine-based organic photovoltaic cells, . X-ray diffraction (PXRD) measurements in order to obtain structural insight into the two components (Fig.

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  • N-Phenyl-1-naphthylamine | CAS 90-30-2 | SCBT - Santa Cruz

    Buy N-Phenyl-1-naphthylamine (CAS 90-30-2), a fluorescent probe, from Santa Cruz Biotechnology. Molecular Formula: C16H13N, Molecular Weight: 219.29.

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  • Affinity of intact Escherichia coli for hydrophobic membrane - PNAS

    philic molecules N-phenyl-1-naphthylamine, pyrene, and 1,6- diphenyl-1 of probe irreversibly bound to the cells. However, in Fluorescence measurements .

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  • Changes in Rotational Motion of a Cell-Bound Fluorophore Caused

    21 Nov 1975 fluorescence of 1 -phenylnaphthylamine in Escherichia coli suspensions were measured before and after addition of colicin. El. Both sets of 

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  • n-phenyl-1-naphthylamine - World Health Organization

    upon studies with Daphnia and fish and its measured of this CICAD is presented in Appendix 2. This CICAD log K of 4.2, N-phenyl-1-naphthylamine is expected 

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  • Rapid production and characterization of antimicrobial colicins using

    In this study, we used cell-free protein synthesis (CFPS) to produce active colicins without the need for protein purifiion and main helps colicins to recognize target cells by binding to a cell . 50mg/ml, as measured by Quick- Start Bradford protein assay uation of N-phenyl-1-naphthylamine as a probe of mem-.

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  • Comparing bacterial membrane interactions and antimicrobial

    cell-selective antimicrobial peptides with improved an- timicrobial LFP-20 in displacing lipopolysaccharide-bound dansyl- ricidal mechanism by measuring depolarization of the β-d-galactoside (ONPG), N-phenyl-1-naphthylamine.

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  • Binding of a fluorescence reporter and a ligand to an odorant - NCBI

    9 Jan 2015 N-phenyl-1-naphthylamine (NPN) is widely used in binding assays with insect OBPs. (5 R,6 Fluorescence measurements were done on a RF-5301 Samples in a 2-ml cell were excited at 337 nm, with the emission spectra 

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  • N-phenyl-1 -naphthylamine as a Fluorescent Probe for Early Event

    8 Jun 1982 N-phenyl-1-naphthylamine — Intensity of fluorescence. Introduction The process of killer toxin action involves binding to a cell wall receptor. (Al-Aidroos and Preparation of cell suspension for fluorescence measurements.

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  • Antimicrobial Properties and Membrane-Active Mechanism of a

    Jan 21, 2014 GI24 resulted in greater cell morphological changes and visible pores on The high net charge may be advantageous to PMAP-36 binding the bacterial . The hemolytic activity of the peptides was measured as the amount of .. was evaluated with the fluorescent dye N-phenyl-1-naphthylamine (NPN).

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  • Interaction of antibacterial compounds with RND efflux - Frontiers

    8 Jul 2015 Hyperexpression of efflux pumps of the Resistance-Nodulation-Cell Division Subtle differences in physicochemical features of compound-pump and The lipophilic membrane partitioning dye N-phenyl-1-naphthylamine (NPN) is useful Rhodamine measurements in P. aeruginosa were sensitive to the 

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  • Antibacterial action of a novel functionalized chitosan-arginine

    11 Jan 2010 Studies using fluorescent probes, 1-N-phenylnaphthylamine (NPN), . chitosan- arginine and the following fluorescence measurement for NPN and NR. .. presence of PI molecules in P. fluorescens cells and binding to DNA 

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